Signosis

Product Name	Catalog #	Price		Qty
NRF2/ARE Luciferase Reporter HepG2 Stable Cell Line	SL-0046-FP	$2,904	X	- +

Description(s):

NRF2/ARE Responsive Luciferase Reporter HepG2 Cell Line is derived from Human Liver cancer, and stably express firefly luciferase reporter gene under the control of NRF2/ARE response element.This cell line is an ideal cellular model for monitoring the activation of Antioxidant response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Principle

NRF2 plays a crucial role in cellular anti-oxidant defense, making it a therapeutic target for neurodegenerative diseases and cancer. Under normal conditions, NRF2 localizes in the cytosol and is rapidly degraded by the proteasome. Under oxidative stress, NRF2 is stabilized and translocates to the nucleus where it binds to a DNA promoter and initiates gene expression. In the nucleus, NRF2 forms a heterodimer with a small Maf protein and binds to the Antioxidant Response Element in the upstream promoter region of many antioxidative genes, and initiates their transcription.

This NRF2 luciferase reporter HepG2 stable cell line has been stably transfected with pTA-ARE-luciferase reporter vector, which contains 4 repeats of antioxidant response binding sites, a minimal promoter upstream of the firefly luciferase coding region, along with a hygromycin expression vector. Following selection, the hygromycin resistant clones were subsequently screened for TBHQ-induced luciferase activity. The clone with the highest fold induction was selected and expanded to produce this stable cell line.

Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation.

Data

Analysis of the NRF2 Pathway Reporter HePG2 Stable Cell Line in response to stimuli. The cells were seeded on a 96-well plate for overnight with DMEM including 10% FBS. The cells then were treated without or with different concentration TBHQ respectively in DMEM and 0.1% FBS for 16 hours. Data was read on a luminometer with a sensitivity of 3x10^-21 moles luciferase.

Literature

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Citations

NRF2/ARE Luciferase Reporter MCF7 Stable Cell Line (SL-0010)

Withaferin A induces Nrf2-dependent protection against liver injury: role of Keap1-independent mechanisms. DL Palliyaguru, DV Chartoumpekis, N Wakabayashi. Free Radical Biology and Medicine 2016. http://dx.doi.org/10.1016/j.freeradbiomed.2016.10.003

Occurrence and in vitro toxicity of unregulated disinfection by-products in two Saskatchewan drinking water treatment plants. Christena Leigh Watts. Handle Proxy, Universidade Da Coruña, 1 Sept. 2018, hdl.handle.net/10388/11499.

Luciferase Stable Expressing Hela Cell Line (SL-0102)

Redox-responsive, reversibly-crosslinked thiolated cationic helical polypeptides for efficient siRNA encapsulation and delivery.N Zheng, Z Song, Y Liu, R Zhang, R Zhang, C Yao. Journal of Controlled Release.Volume 205, 10 May 2015, Pages 231–239

Targeted delivery of cisplatin to tumor xenografts via the nanoparticle component of nano-diamino-tetrac. Thangirala Sudha, Dhruba J Bharali, Murat Yalcin, Noureldien HE Darwish, Melis Debreli Coskun, Kelly A Keating, Hung-Yun Lin, Paul J Davis, Shaker A Mousa. February 2017 ,Vol. 12, No. 3, Pages 195-205 , DOI 10.2217/nnm-2016-0315.

NFkB Luciferase Reporter Stable Cell Lines (SL-0001)

Thymoquinone Modulates Blood Coagulation in Vitro via Its Effects on Inflammatory and Coagulation Pathways. V Muralidharan-Chari, J Kim, A Abuawad, M Naeem. Int. J. Mol. Sci. 2016, 17(4), 474; doi:10.3390/ijms17040474

Drug repurposing screen identifies lestaurtinib amplifies the ability of the poly (ADP-ribose) polymerase 1 inhibitor AG14361 to kill breast cancer associated gene-1 …G Vazquez-Ortiz, C Chisholm, X Xu, TJ Lahusen, C Li… - Breast Cancer Research 2014, Jun 24;16(3):R67. doi: 10.1186/bcr3682.

NFkB Luciferase Reporter A549 Stable Cell Lines (SL-0014)

Alcohol promotes migration and invasion of triple-negative breast cancer cells through activation of p38 MAPK and JNK†M Zhao, EW Howard, AB Parris, Z Guo, Q Zhao, X Yang. Molecular Carcinogenesis, 2016, DOI: 10.1002/mc.22538

HEK293 TCF/LEF Luciferase Reporter Stable Cell Line (SL-0015)

Active β-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A. Amit Persad,1,* Geetha Venkateswaran,1,* Li Hao,1 Maria E. Garcia,1 Jenny Yoon,1 Jaskiran Sidhu,1 and Sujata Persad1. Genes Cancer. 2016 Nov; 7(11-12): 368–382., doi: 10.18632/genesandcancer.128

Diallyl trisulfide inhibits proliferation, invasion and angiogenesis of glioma cells by inactivating Wnt/β-catenin signaling. Qingxia Tao, Cuiying Wu, Ruxiang Xu, Lijun Niu, Jiazhen Qin, Ning Liu, Peng Zhang, Chong Wang. Cell and Tissue Research, 2017, doi:10.1007/s00441-017-2678-9.

BCN057 induces intestinal stem cell repair and mitigates radiation-induced intestinal injury. Payel Bhanja, Andrew Norris, Pooja Gupta-Saraf, Andrew Hoover and Subhrajit Saha. Stem Cell Research & Therapy, vol. 9, no. 1, Feb. 2018, doi:10.1186/s13287-017-0763-3.

(Nrf2/ARE) luciferase reporter cell line HEK293 (SL-0042)

Direct Antioxidant Properties of Methotrexate: Inhibition of Malondialdehyde-Acetaldehyde-Protein Adduct Formation and Superoxide Scavenging. Matthew C. Zimmermana, Dahn L. Clemensb, c, Michael J. Duryeeb, Cleofes Sarmientoa, Andrew Chioub, Carlos D. Hunterb, Jun Tiana, Lynell W. Klassenb, c, James R. O’Dellb, c, Geoffrey M. Thieleb, c, Redox Biology,2017.07.018, doi: 10.1016/j.redox.2017.07.018

Active β-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A. Amit Persad1,*, Geetha Venkateswaran1,*, Li Hao1, Maria E. Garcia1, Jenny Yoon1, Jaskiran Sidhu1 and Sujata Persad1.Genes & Cancer. January 30, 2017 . https://doi.org/10.18632/genesandcancer.128

HIF Luciferase Reporter Hela Stable Cell Line (SL-0023)

Hypothermia augments stress response in mammalian cells. Eskla, Kattri-Liis, et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.04.571.

NFkB Luciferase Reporter HepG2 Stable Cell Line (SL-0017)

Hypothermia augments stress response in mammalian cells. Eskla, Kattri-Liis, et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.04.571.

CHOP Luciferase Reporter Mia_Paca2 Stable Cell Line (SL-0025)

A new ent-norabietant diterpenoid from roots of Euphorbia lathyris L. Yun, Young Sook, et al. Tetrahedron Letters, 2018, doi:10.1016/j.tetlet.2018.06.017.

NFAT Luciferase Reporter Jurkat Stable Cell Line (SL-0032)

Diversification of Bw4 Specificity and Recognition of a Nonclassical MHC Class I Molecule Implicated in Maternal–Fetal Tolerance by Killer Cell Ig-like Receptors of the Rhesus Macaque. Banerjee, Priyankana, et al. The Journal of Immunology, 2018, doi:10.4049/jimmunol.1800494.

P329G-CAR-J: a Novel Jurkat-NFAT-Based CAR-T Reporter System Recognizing the P329G Fc Mutation. Darowski, Diana, et al. Protein Engineering, Design and Selection, 2019, doi:10.1093/protein/gzz027.

Dendritic cells dictate responses to PD-L1 blockade cancer immunotherapy. Mayoux, Maud, et al. Science Translational Medicine Vol. 12, Issue 534, 2020.
DOI: 10.1126/scitranslmed.aav7431

NRF2/ARE Luciferase Reporter HepG2 Stable Cell Line (SL-0046)

Innovative drinking water treatment techniques reduce the disinfection-induced oxidative stress and genotoxic activity. Lundqvist, Johan, et al. Water Research, vol. 155, 2019, pp. 182–192., doi:10.1016/j.watres.2019.02.052.

Stat3 Luciferase Reporter Hela Stable Cell Line (SL-0003)

Discovery of a New STAT3 Inhibitor Acting on the Linker Domain. Koseki, Tatsuya, et al. Biological and Pharmaceutical Bulletin, vol. 42, no. 5, 2019, pp. 792–800., doi:10.1248/bpb.b18-00992.

Stat1 Luciferase Reporter Hela Stable Cell Line (SL-0004)

Discovery of a New STAT3 Inhibitor Acting on the Linker Domain. Koseki, Tatsuya, et al. Biological and Pharmaceutical Bulletin, vol. 42, no. 5, 2019, pp. 792–800., doi:10.1248/bpb.b18-00992.

NRF2/ARE Luciferase Reporter NIH3T3 Stable Cell Line (SL-0047)

Thiamine and Benfotiamine Protect Neuroblastoma Cells against Paraquat and β-Amyloid Toxicity by a Coenzyme-Independent Mechanism. Sambon, Margaux, et al. Heliyon, vol. 5, no. 5, 2019, doi:10.1016/j.heliyon.2019.e01710.

NFkB Luciferase Reporter HEK293 Stable Cell Line (SL-0012)

Vitale LA, He LZ, Thomas LJ, et al. Development of CDX-527: a bispecific antibody combining PD-1 blockade and CD27 costimulation for cancer immunotherapy. Cancer Immunology, Immunotherapy : CII. 2020 May. DOI: 10.1007/s00262-020-02610-y.

Luciferase Substrate (LUC15/100)

FXR Luciferase Reporter HepG2 Stable Cell Line (SL-0055)

Targeting the Liver‐Brain Axis with Hop‐Derived Flavonoids Improves Lipid Metabolism and Cognitive Performance in Mice. Paraiso, I.L., Revel, J.S., Choi, J., Miranda, C.L., Lak, P., Kioussi, C., Bobe, G., Gombart, A.F., Raber, J., Maier, C.S. and Stevens, J.F. Mol. Nutr. Food Res. 2020, 2000341. https://doi.org/10.1002/mnfr.202000341

Estrogen Receptor Luciferase Reporter T47D Stable Cell Line (SL-0002)

Glass-bottled drinking water: a time capsule to study the historic presence of hazardous chemicals using effect-based methods. Lundqvist, J., Persson, K.M. & Oskarsson, A. Environ Sci Eur 33, 34 (2021). https://doi.org/10.1186/s12302-021-00476-0