Description(s):
The Nuclear Extraction Kit is used for preparation of nuclear extracts, which often need for studying transcription factors. The Nuclear Extraction Kit contains reagents for the preparation of 200 nuclear extracts from culture cells in 6-well plate and 50 nuclear extracts from cultured cells in 100-mm culture dishes. The kit can also be used for nuclear extraction of soft tissue samples (brain, kidney, liver, etc), however there may be additional steps required to release cells from tissues.
Literature
View user manualCitations
1.Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor-kappa B pathway in neuroglioma cells L. Zhang, J. Zhang, L. Yang, Y. Dong, Y. Zhang and Z. Xie. Br. J. Anaesth. (2013). doi: 10.1093/bja/aet115.
2.Enforced expression of Lin28b leads to impaired T-cell development, release of inflammatory cytokines and peripheral T-cell lymphoma Beachy SH, Onozawa M, Chung YJ, Slape C, Bilke S, Francis P, Pineda M, Walker RL, Meltzer P, Aplan PD. Blood. 2012 Aug 2;120(5):1048-59.
3.Immunoglobulin light chains activate nuclear factor-B in renal epithelial cells through a Src-dependent mechanism Ying WZ, Wang PX, Aaron KJ, Basnayake K, Sanders PW. Blood. 2011 Jan 27;117(4):1301-7.
4.Adipose Tissue-specific Inhibition of Hypoxia-inducible Factor 1 Induces Obesity and Glucose Intolerance by Impeding Energy Expenditure in Mice Zhang X, Lam KS, Ye H, Chung SK, Zhou M, Wang Y, Xu A. J Biol Chem. 2010 Oct 22;285(43):32869-77.
5.Septic shock NTIS causes hypothyroidism and conditions for reducedsensitivity to thyroid hormone. Castro I, Quisenberry L, Calvo RM, Obregon MJ, Lado-Abeal J. J Mol Endocrinol. 2013 Mar 18;50(2):255-66. doi: 10.1530/JME 12-0188. Print 2013.
6.Molecular mechanism depressing PMA-induced invasive behaviors in human lung adenocarcinoma cells by cis- and trans-cinnamic acid. Tsai CM, Sun FM, Chen YL, Hsu CL, Yen GC, Weng CJ. Eur J Pharm Sci. 2012 Dec 7;48(3):494-501. doi: 10.1016/j.ejps.2012.11.013. [Epub ahead of print]
7.Protein kinase C inhibitor AEB071 targets ocular melanoma harboring GNAQ mutations via effects on the PKC/Erk1/2 and PKC/NF-κB pathways Wu X, Li J, Zhu M, Fletcher JA, Hodi FS. Mol Cancer Ther. 2012 Sep;11(9):1905-14. doi: 10.1158/1535-7163.MCT-12-0121. Epub 2012 May 31.
8.Oncogenic KRAS Impairs EGFR Antibodies' Efficiency by C/EBPβ-Dependent Suppression of EGFR Expression1,2 Derer S, Berger S, Schlaeth M, Schneider-Merck T, Klausz K, Lohse S, Overdijk MB, Dechant M, Kellner C, Nagelmeier I, Scheel AH, Lammerts van Bueren JJ,van de Winkel JG, Parren PW, Peipp M, Valerius T. Neoplasia. 2012 Mar;14(3):190-205.
9.Differential Regulation Of Micrornas In Asthmatic Airways Le W, Ghani M, Gutsche M, Wang W, Hwang PH, Upadhyay D Am. J. Respir. Crit. Care Med., 2011 May;183: A2067.
10.MicroRNAs in resolution of acute inflammation: identification of novel resolvin D1-miRNA circuits Recchiuti A, Krishnamoorthy S, Fredman G, Chiang N, Serhan CN. FASEB J. 2011 Feb;25(2):544-60.
11. The secondary bile acid, deoxycholate accelerates intestinal adenoma–adenocarcinoma sequence in Apc min/+ mice through enhancing Wnt signaling. H Cao, S Luo, M Xu, Y Zhang, S Song, S Wang, X Kong… - Familial Cancer, 2014
12.Erythropoietin Delivered via Intra-Arterial Infusion Reduces Endoplasmic Reticulum Stress in Brain Microvessels of Rats Following Cerebral Ischemia and Reperfusion. Journal of Neuroimmune …, 2015
13. The handedness-associated PCSK6 locus spans an intronic promoter regulating novel transcripts. R Shore, L Covill, KA Pettigrew, WM Brandler, R Diaz Human Molecular Genetics, 2016 HMG Advance Access published February 21, 2016
14. MGMT DNA repair gene promoter/enhancer haplotypes alter transcription factor binding and gene expression
M Xu, CE Cross, JT Speidel, SZ Abdel-Rahman doi:10.1007/s13402-016-0286-4 Cellular Oncology, 2016
15. Vitamin D3 Partly Antagonizes Advanced-Glycation Endproducts-Induced NFκB Activation in Mouse Podocytes. Rüster C. · Franke S. · Reuter S. · Mrowka R. · Bondeva T. · Wolf G. Department of Internal Medicine III, University Hospital Jena, Jena, Germany . Nephron 2016 (DOI:10.1159/000448106)
16. Secondary bile acid‐induced dysbiosis promotes intestinal carcinogenesis. Hailong Cao, Mengque Xu, Wenxiao Dong, Baoru Deng, Sinan Wang, Yujie Zhang, Shan Wang, Shenhui Luo, Weiqiang Wang, Yanrong Qi, Jianxin Gao, Xiaocang Cao, Fang Yan, Bangmao Wang. International Journal of Cancer 2017 (DOI: 10.1002/ijc.30643)
17. A Novel Redox Regulator, MnTnBuOE-2-PyP5+, Enhances Normal Hematopoietic Stem/Progenitor Cell Function. Y. Zhaoa, D.W Carrolla, Y. Youb, L. Chaiswinga, R. Wend, I. Batinic-Haberlec, S. Bondadae, Y. Lianga, D.K. St. Clair. Redox Biology 2017 (http://dx.doi.org/10.1016/j.redox.2017.02.005)
18. Plasmodium falciparum PfEMP1 modulates monocyte/macrophage transcription factor activation, and cytokine and chemokine responses. Natália Guimarães Sampaio, Emily Marie Erikssona, and Louis Schofield. Infection and Immunity, 2017, doi:10.1128/iai.00447-17.
19. Promoter Haplotypes of the ABCB1 Gene Encoding the P-Glycoprotein Differentially Affect Its Promoter Activity by Altering Transcription Factor Binding. Speidel, Jordan T., et al. DNA and Cell Biology, 2018, doi:10.1089/dna.2018.4376.
20. Interleukin-18 Binding Protein Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Suppression NF-ΚB and Activation Nrf2 Pathway. Zhang, Li-Ming, et al. Biochemical and Biophysical Research Communications, 2018, doi:10.1016/j.bbrc.2018.09.193.
21. Gong-Ping Liu, Xiao-Yue Hong, Hua-Li Wan et al. STAT3 ameliorates cognitive deficits by positively regulating the expression of NMDARs in a mouse model of FTDP-17, 07 May 2020, PREPRINT (Version 1) available at Research Square [+https://doi.org/10.21203/rs.3.rs-25793/v1+]
22. Rieske iron-sulfur protein induces FKBP12.6/RyR2 complex remodeling and subsequent pulmonary hypertension through NF-κB/cyclin D1 pathway. Mei, L., Zheng, Y., Song, T. et al. Nat Commun 11, 3527 (2020). https://doi.org/10.1038/s41467-020-17314-1
23. Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits. Yao, J., Zhao, X., Tan, F. et al. Sci Rep 10, 11638 (2020). https://doi.org/10.1038/s41598-020-68543-9
24. Sirt1 and Sirt3 Activation Improved Cardiac Function of Diabetic Rats via Modulation of Mitochondrial Function. Paramesha, B.; Anwar, M.S.; Meghwani, H.; Maulik, S.K.; Arava, S.K.; Banerjee, S.K. Antioxidants; Basel Vol. 10, Iss. 3, (2021): 338. https://doi.org/10.3390/antiox10030338
25. Singular and short-term anesthesia exposure in the developing brain induces persistent neuronal changes consistent with chronic neurodegenerative disease. Hogarth, K., Vanama, R.B., Stratmann, G. et al. Sci Rep 11, 5673 (2021). https://doi.org/10.1038/s41598-021-85125-5
26. Inflammatory signaling sensitizes Piezo1 mechanotransduction in articular chondrocytes as a pathogenic feed-forward mechanism in osteoarthritis. Whasil Lee, Robert J. Nims, Alireza Savadipour, Qiaojuan Zhang, Holly A. Leddy, Fang Liu, Amy L. McNulty, Yong Chen, Farshid Guilak, Wolfgang B. Liedtke. Proceedings of the National Academy of Sciences Mar 2021, 118 (13) e2001611118; DOI: 10.1073/pnas.2001611118
27. Channeling macrophage polarization by rocaglates increases macrophage resistance to Mycobacterium tuberculosis. Chatterjee, S., Yabaji, S.M., Rukhlenko, O.S., Bhattacharya, B., Waligurski, E., Vallavoju, N., Ray, S., Kholodenko, B.N., Brown, L.E., Beeler, A.B., Ivanov, A.R., Kobzik, L., Porco Jr., J.A., Kramnik, I., ISCIENCE (2021), doi: https://doi.org/10.1016/j.isci.2021.102845.
28. Furtek, Steffanie L., et al. "Evaluation of quantitative assays for the identification of direct signal transducer and activator of transcription 3 (STAT3) inhibitors." Oncotarget [Online], 7.47 (2016): 77998-78008. Web. 2 Feb. 2023
