Characterization
Description(s):Two key transcription factors, Oct4 and Sox2, have been identified that are crucial for maintenance of the pluripotent state of ESCs. Octamer 3/4 (Oct 3/4), a member of the POU family, is considered to be an important stem cell marker and essential transcription factor during human embryogenesis. Sox2 is a member of the Sox (SRY-Related HMG box) gene family that encodes transcription factors with a single HMG DNA-binding domain. Sox2 controls neural progenitor cells by prohibiting their ability to differentiate. In recent years, there have also been reports about the presence of Oct 3/4 and Sox2 in differentiated benign and malignant human cells. Oct4 and Sox2 showed cooperative binding to the Sox-Oct motifs on some target promoter regions. In addition, the transcription factor Nanog, an established regulator of pluripotency, is transcriptionally regulated directly by Oct4 and Sox2.
Applicable Grid:
OCT4 and SOX2 ELISA Kits Available:
Principle
TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.
Data
OCT4 TF ELISA Kit analysis of OCT4 Activation in F9 nuclear extract. The F9 nuclear extracts with a series of dilution were incubated on the OCT4 plate coated with OCT4 binding consensus sequence respectively. The bound OCT4 was detected with mouse anti-OCT4 antibody.
