Product Name Catalog # Price   Qty
Oxidative Stress TF Activation Profiling Plate Array FA-1005 $801
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Characterization

Description(s):

Oxidative stress occurs when the steady-state balance between pro-oxidant and anti-oxidant is changed, and oxidative stress has been reported to associate with many diseases, such as cancer, Alzheimer's disease, Parkinson's disease, atherosclerosis, and heart failure. Responses to oxidative stress are mediated by a large number of transcription factors (TFs).

Signosis has developed the Oxidative Stress TF Activation Profiling Plate Array, which can be used to simultaneously monitor 16 oxidative stress related TFs, including AP1, ATF4, CBF/NFY, EGR1, ETS, FOXO1, HIF, HSF, IRF, NFAT, NFkB, NRF2/ARE, p53, Smad, SRF, and Stat3. 


Applicable Grid:

List of Applicable TFs:

  1 2 3 4 5 6 7 8 9 10 11 12
A AP1 IRF AP1 IRF AP1 IRF AP1 IRF AP1 IRF AP1 IRF
B ATF4 NFAT ATF4 NFAT ATF4 NFAT ATF4 NFAT ATF4 NFAT ATF4 NFAT
C CBF/NFY NFkB CBF/NFY NFkB CBF/NFY NFkB CBF/NFY NFkB CBF/NFY NFkB CBF/NFY NFkB
D EGR1 NRF2/ARE EGR1 NRF2/ARE EGR1 NRF2/ARE EGR1 NRF2/ARE EGR1 NRF2/ARE EGR1 NRF2/ARE
E ETS P53 ETS P53 ETS P53 ETS P53 ETS P53 ETS P53
F FOXO1 Smad FOXO Smad FOXO Smad FOXO Smad FOXO Smad FOXO Smad
G HIF SRF HIF SRF HIF SRF HIF SRF HIF SRF HIF SRF
H HSF Stat3 HSF Stat3 HSF Stat3 HSF Stat3 HSF Stat3 HSF Stat3

 


Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.

 

 

Literature

View user manual

Citations

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