Description(s):

Stable cell lines are great tool for the study of gene regulation and cellular signaling pathways. Signosis over the last few years has successfully developed  many different stable cell lines that are applicable for a wide range of research applications and drug discovery. Here Signosis offer some of the plasmids used in generating those stable cell lines.

 

Highlight

• Panel of EGFR Mutant Vectors  - The panel includes more than ready-to-go EGFR mutants.
• DYKDDDDK-tag for easy detection - This tag is engineered to downstream of EGFR gene to facilitate Western blot
• Stable cell line establishment-  The hygromycin resistant gene is embedded in the vector for stable cell line establishment.
• Custom service available - We also offer the custom service with the same list price. If you have any mutants in your mind, please let us know. 

Principle

Mutations in the epidermal growth factor receptor (EGFR) gene are commonly observed in many type of cancers including lung, colorectal, head and neck and breast cancer. These mutations play an important role in particular in non-small-cell lung cancer as they can be not only therapeutic target but also a predictive tool for response to EGFR tyrosine kinase inhibitors such as gefitinib and erlotinib. Cell lines have played crucial roles in the identification and characterization of these driver mutations. Signosis has developed expression vectors for the most common EGFR mutations harboring FLAG marker with high expression suitable for your cancer research. We also accept any other customized EGFR mutation for your research need, contact us for more information.