Description(s):

The plenti-EGFR (L718Q, L858R) vector contains EGFR with the L718Q, L858R mutations under the MSCV promoter which can be used to infect both dividing and nondividing cells. It contains the HIV-1 LTRs and the lentiviral packaging signal (Ψ) for retroviral expression, with specific elements (WPRE, cPPT, RRE) included for improved transgene expression. Downstream of the EGFR viii gene is a DYKDDDDK-tag for Western blotting. Furthermore, copGFP can be used as a selection marker when used with stable cell lines.

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death.  The EGFR L718Q, L858R mutations involve point mutations that changes a L (Leu) into an Q (Gln) at point 718, and a L into an R (Arg) at point 858.

 

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.