Principle
The specific miRNA complementary sequence has been engineered at 3' untranslated region of luciferase gene to make a miRNA luciferase reporter vector. When miRNA targets the binding site at 3'UTR downstream of reporter luciferase gene, it leads to repression of the expression of luciferase and results in reduction of luciferase enzyme activity. Through comparison of luciferase activity in two samples, up- or down-regulated miRNA can be quantitatively measured.
