Description(s):

The pMSCV-EGFR wt vector contains the wt EGFR gene under the MSCV promoter for high expression in hematopoietic, HEK293, and stem cells. Downstream of the EGFR gene is a DYKDDDDK-tag for Western blotting. Furthermore, the coGFP under the EF1 promoter can be used as a selection marker with stable cell lines. The base vector is a modified pMSCV vector with the LTRs and the lentiviral packaging signal (Ψ) removed for standard expression. For retroviral expression systems, please see our ELM series or contact customer support.

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand binding domain and exons 18–24 code for the TK domain.  Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT and JAK/STAT pathways which can lead to cell survival, proliferation and migration of cancer cells.

 

 

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.