Description(s):
NFkB reporter vector is utilized for detecting NFkB (Nuclear factor of kappa light polypeptide gene) transactivation in a cell-based assay. The vector has been functionally validated in cells with high induction fold upon treatment.
Principle
The Luciferase Reporter Vectors have been specially constructed to report the binding activity of a single TF. Multiple copies (at least four) of the cis-acting enhancer element have been inserted into each vector upstream of a minimal TA promoter. Upon vector transfection and cell stimulation, the activated TF is bound to the enhancer element and initiate the transcription of downstream luciferase reporter gene. The chemiluminescent measurement of luciferase activity is proportional to the amount of expressed enzyme and thus the binding activity of the targeted TF.
Data
Citations
| Apoptosis Luciferase Reporter Vector Set LR-3002 |
| Expression of the multidrug transporter P-glycoprotein is inversely related to that of apoptosis-associated endogenous TRAIL PS Souza, JP Madigan, JP Gilleta, K Kapoor… - Experimental Cell Research, 2015 Available online 19 June 2015. |
| pSTAT1 -Luc LR-2003 |
| EZH2-Mediated Inactivation of IFN-g-JAK-STAT1 Signaling Is an Effective Therapeutic Target in MYC-Driven Prostate Cancer.Zhen Ning,Wee,1,3 Zhimei Li,1 Puay Leng Lee,1 Shuet Theng Lee,1 ... Cell Reports Article. |
| pSTAT3-Luc LR-2004 |
| Impact of Acetazolamide, a Carbonic Anhydrase Inhibitor, on the Development of Intestinal Polyps in Min Mice. Nobuharu Noma, Gen Fujii, Shingo Miyamoto, Masami Komiya, Ruri Nakanishi, Misato Shimura, Sei-ichi Tanuma and Michihiro Mutoh. International Journal of Molecular Sciences 18.4 (2017). doi: 10.3390/ijms18040851. |
| pAP1-Luc (LR-2006) / pNRF2/ARE-Luc (LR-2106) / pp53-Luc (LR-2041) / Pta-Luc (LR-2200) |
| Impact of Acetazolamide, a Carbonic Anhydrase Inhibitor, on the Development of Intestinal Polyps in Min Mice. Nobuharu Noma, Gen Fujii, Shingo Miyamoto, Masami Komiya, Ruri Nakanishi, Misato Shimura, Sei-ichi Tanuma and Michihiro Mutoh. International Journal of Molecular Sciences 18.4 (2017). doi: 10.3390/ijms18040851. |
| Nrf2/ARE pathway attenuates oxidative and apoptotic response in human osteoarthritis chondrocytes by activating ERK1/2/ELK1-P70S6K-P90RSK signaling axis. Khan, Nazir M., et al. Free Radical Biology and Medicine, 2018, doi:10.1016/j.freeradbiomed.2018.01.013. |
| Pstat4 LR-2005 |
|
The Regulatory Function of miR-200c on Inflammatory and Cell-Cycle Associated Genes in SK-LMS-1, A Leiomyosarcoma Cell Line. TD Chuang, M Ho, O Khorram - Reproductive Sciences, 2014. |
| pNFKB-Lu LR-2001 |
| Analysis of CARD14 Polymorphisms in Pityriasis Rubra Pilaris: Activation of NF-κB. V Ratushny, GP Smith – analysis. Journal of Investigative Dermatology(2015)135,1905–1908; doi:10.1038/jid.2015.65; published online 2 April 2015. |
| The Regulatory Function of miR-200c on Inflammatory and Cell-Cycle Associated Genes in SK-LMS-1, A Leiomyosarcoma Cell Line. TD Chuang, M Ho, O Khorram - Reproductive Sciences, 2014. |
| Angiotensin II Differentially Regulates Morg1 Expression in Kidney Cells.Bondeva T, Heinzig J, Franke S, Wolf G.Am J Nephrol. 2012;35(5):442-55. doi: 10.1159/000337922. Epub 2012 Apr 27. |
| Endometrial miR-200c is Altered During Transformation into Cancerous States and Targets the Expression of ZEBs, VEGFA, FLT1, IKKβ, KLF9, and FBLN5. Panda H, Pelakh L, Chuang TD, Luo X, Bukulmez O, Chegini N. Reprod Sci. 2012 Aug;19(8):786-96. |
| The Regulatory Function of miR-200c on Inflammatory and Cell-Cycle Associated Genes in SK-LMS-1, A Leiomyosarcoma Cell Line. TD Chuang, M Ho, O Khorram - Reproductive Sciences, 2014. |
| Evidence for a genomic mechanism of action for progesterone receptor membrane component-1. Peluso JJ, Decerbo J, Lodde V. Steroids. 2012 Aug;77(10):1007-12. |
| The Nrf2 activator, tBHQ, differentially affects early events following stimulation of Jurkat cells. Toxicol. Sci. (2013) doi: 10.1093/toxsci/kft172 First published online: August 14, 2013. |
| Impact of Acetazolamide, a Carbonic Anhydrase Inhibitor, on the Development of Intestinal Polyps in Min Mice. Nobuharu Noma, Gen Fujii, Shingo Miyamoto, Masami Komiya, Ruri Nakanishi, Misato Shimura, Sei-ichi Tanuma and Michihiro Mutoh. International Journal of Molecular Sciences 18.4 (2017). doi: 10.3390/ijms18040851. |
| pOct-1-Luc reporter vector LR-2063 |
| Prelamin A accumulation and stress conditions induce impaired Oct-1 activity and autophagy in prematurely aged human mesenchymal stem cell. A Infante, A Gago, GR de Eguino, T Calvo-Fernández… - AGING, 2014. |
| Double Suppression of the Gα Protein Activity by RGS Proteins. Lin C, Koval A, Tishchenko S, Gabdulkhakov A, Tin U, Solis GP, Katanaev VL. Mol Cell. 2014 Feb 20;53(4):663-71. |
| Dicer Knockdown Inhibits Endothelial Cell Tumor Growth via MicroRNA 21a-3p Targeting of Nox-4. Gordillo GM, Biswas A, Khanna S, Pan X, Sinha M, Roy S, Sen CK. J Biol Chem. 2014 Feb 4. |
| Transient Maternal Hypothyroxinemia Potentiates the Transcriptional Response to Exogenous Thyroid Hormone in the Fetal Cerebral Cortex Before the Onset of Fetal Thyroid Function: A Messenger and MicroRNA Profiling Study. Dong H, You SH, Williams A, Wade MG, Yauk CL, Thomas Zoeller R. Cereb Cortex. 2014 Feb 7. |
| Endothelin-1 Induces a Glycolytic Switch in Pulmonary Arterial Endothelial Cells via the Mitochondrial Translocation of Endothelial NO Synthase. Sun X, Kumar S, Sharma S, Aggarwal S, Lu Q, Gross C, Rafikova O, Lee SG, Dasarathy S, Hou Y, Meadows ML, Han W, Su Y, Fineman JR, Black SM. Am J Respir Cell Mol Biol. 2014 Jan 6. |
| Common food additive carrageenan stimulates Wnt/ β-catenin signaling in colonic epithelium by inhibition of nucleoredoxin reduction. Bhattacharyya S, Feferman L, Borthakur S, Tobacman JK. Nutr Cancer. 2014 ;66(1):117-27. |
| Effects of all-trans retinoic acid, retinol, and β-carotene on murine macrophage activity. Lo HM, Wang SW, Chen CL, Wu PH, Wu WB. Food Funct. 2014 Jan;5(1):140-8. |
| The Nrf2 activator, tBHQ, differentially affects early events following stimulation of Jurkat cells. Zagorski JW, Turley AE, Dover HE, VanDenBerg KR, Compton JR, Rockwell CE. Toxicol Sci. 2013 Nov;136(1):63-71. |
| Selectively Targeting Prostate Cancer with Antiandrogen Equipped Histone Deacetylase Inhibitors. Gryder BE, Akbashev MJ, Rood MK, Raftery ED, Meyers WM, Dillard P, Khan S, Oyelere AK. ACS Chem Biol. 2013 Sep 20. [Epub ahead of print] |
| Advanced glycated end products affect HIF- transcriptional activity in renal cells. Bondeva T, Heinzig J, Ruhe C, Wolf G. Mol Endocrinol. 2013 Sep 12. [Epub ahead of print] |
| Obesity induced a leptin-Notch signaling axis in breast cancer. Battle M, Gillespie C, Quarshie A, Lanier V, Harmon T, Wilson K, Torroella-Kouri M, Gonzalez-Perez RR. Int J Cancer. 2013 Sep 30. [Epub ahead of print] |
| The carotenoid lutein enhances matrix metalloproteinase-9 production and phagocytosis through intracellular ROS generation and ERK1/2, p38 MAPK, and RARβ activation in murine macrophages. Lo HM, Chen CL, Yang CM, Wu PH, Tsou CJ, Chiang KW, Wu WB. J Leukoc Biol. 2013 May;93(5):723-35. |
| Angiotensin II Differentially Regulates Morg1 Expression in Kidney Cells. Bondeva T, Heinzig J, Franke S, Wolf G. Am J Nephrol. 2012 ;35(5):442-55. |
| Endometrial miR-200c is Altered During Transformation into Cancerous States and Targets the Expression of ZEBs, VEGFA, FLT1, IKKβ, KLF9, and FBLN5. Panda H, Pelakh L, Chuang TD, Luo X, Bukulmez O, Chegini N. Reprod Sci. 2012 Aug;19(8):786-96. |
| Evidence for a genomic mechanism of action for progesterone receptor membrane component-1. Peluso JJ, Decerbo J, Lodde V. Steroids. 2012 Aug;77(10):1007-12. |
| The histone deacetylase SIRT6 is a tumor suppressor that controls cancer metabolism. Sebastián C, Zwaans BM, Silberman DM, Gymrek M, Goren A, Zhong L, Ram O, Truelove J, Guimaraes AR, Toiber D, Cosentino C, Greenson JK, MacDonald AI, McGlynn L, Maxwell F, Edwards J, Giacosa S, Guccione E, Weissleder R, Bernstein BE, Regev A, Shiels PG, Lombard DB, Mostoslavsky R. Cell 2012 41615;151(6):1185-99. |
| Influences of flavones on cell viability and cAMP-dependent steroidogenic gene regulation in MA-10 Leydig cells. M Cormier, F Ghouili, P Roumaud, W Bauer… - Cell Biology and Toxicology ,28 April 2017;DOI: 10.1007/s10565-017-9395-8. |
| Glucocorticoids regulate MiR-29c levels in vascular smooth muscle cells through transcriptional and epigenetic mechanisms. TD Chuang, O Khorram - Life Sciences, Volume 186, 1 October 2017, Pages 87-91. https://doi.org/10.1016/j.lfs.2017.08.007 |
| Active β-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A. Amit Persad1,*, Geetha Venkateswaran1,*, Li Hao1, Maria E. Garcia1, Jenny Yoon1, Jaskiran Sidhu1 and Sujata Persad1.Genes & Cancer. January 30, 2017 . https://doi.org/10.18632/genesandcancer.128 |
| pAP1-Luc (LR-2006) |
| ATF2 promotes urothelial cancer outgrowth via cooperation with androgen receptor signaling. Inoue, Satoshi, et al. Endocrine Connections, 2018, pp. 1397–1408., doi:10.1530/ec-18-0364. |
| PXR Functionally Interacts with NF-κB and AP-1 to Downregulate the Inflammation-Induced Expression of Chemokine CXCL2 in Mice. Okamura, Maya and Shizu, Ryota and Abe, Taiki and Kodama, Susumu and Hosaka, Takuomi and Sasaki, Takamitsu and Yoshinari, Kouichi. Cells, 2020;10(9):2296, ISSN: 2073-4409, DOI: 10.3390/cells9102296 |
| pAP1-Luc (LR-2006) and NFkB-Luc (LR-2001) |
| Platonin, a Cyanine Photosensitizing Dye, Ameliorates Inflammatory Responses in Vascular Smooth Muscle Cells by Modulating Inflammatory Transcription Factors. Chiu, C.-W.; Yang, C.-H.; Tsai, J.-H.; Hsieh, C.-Y.; Huang, S.-Y. Applied Sciences. 2021; 11(3):1130. https://doi.org/10.3390/app11031130. |
