Characterization

Description(s):

Peroxisome-proliferator-activated receptor gamma (PPAR gamma) is a ligand-activated transcription factor that plays an important role in the control of gene expression associated with a variety of physiological processes, particularly in metabolism and adipogenesis.  With ligand binding, PPAR gamma forms a heterodimer with the retinoic X receptor, and binds to PPAR response elements in the promoter region of target genes, thus regulating their expression. Dysfunction of PPAR gamma leads to the pathological processes, such as metabolic diseases and cancer. Furthermore, modulation of receptor action in these diseases may be of therapeutic value. PPARgamma has been demonstrated to be the target of the thiazolidinediones (TZDs), which are widely used to treat type 2 diabetes. Signosis has developed PPARgamma ELISA, to help specific and sensitive measurement of the activation of PPARgamma.

Applicable Grid:

PPAR gamma ELISA Kits Available:

PPAR gamma ELISA Kit (Colorimetric) TE-0009

PPAR gamma ELISA Kit (Chemiluminescence) TE-0010

 

Principle

TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.

 

Literature

View user manual

Citations

1. Ambroxol improves lysosomal biochemistry in glucocerebrosidase mutation-linked Parkinson disease cells. McNeill A, Magalhaes J, Shen C, Chau KY, Hughes D, Mehta A, Foltynie T, Cooper JM, Abramov AY, Gegg M, Schapira AH. Brain. 2014 Feb 25. [Epub ahead of print]
 
2.Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ. B Rahardjo, E Widjajanto, H Sujuti, K Keman - Biomarkers and Genomic Medicine, 2014
  
3.The secondary bile acid, deoxycholate accelerates intestinal adenoma–adenocarcinoma sequence in Apcmin/+ mice through enhancing Wnt signaling. H Cao, S Luo, M Xu, Y Zhang, S Song, S Wang, X Kong… - Familial Cancer, 2014