Characterization
Description(s):Angiogenesis shifted from the avascular to vascular states is a key event for sustained tumor growth and cancer progression. Angiogenesis as a biological switch process is governed by numerous pro- and anti-angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGFb) and IL-6. The mechanism of action of each of these factors is different, as are their origin and the stimuli for their production. The angiogenic switch refers to the balance between pro- and anti- angiogenic factors. Therefore, profiling of these factors is critical to understanding angiogenesis. Signosis’ Rat Angiogenesis ELISA Strip Profiling Assay allows simultaneously profiling 8 rat angiogenesis cytokines. The difference of these proteins between two samples can be determined through data comparison.
Detection Range:
2 - 2000 pg/mL
Sensitivity:
1 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines
| TNFα | VEGF | IL-6 | FGFb | IFNγ | Leptin | MCP-1 | Rantes |
Principle
Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, eight different proteins can be measured simultaneously. The test sample initially reacts with the solid phase capture antibody, resulting in the cytokine being bound to the well. The wells are then washed to remove unbound proteins, and biotin-linked antibodies are added to the samples to bind to the cytokines. After washing away the unbound antibodies, Streptavidin-HRP conjugate is added to the sample to form a complex with the antibody-bound cytokines. After incubation, the wells are washed to remove unbound Streptavidin-HRP conjugate. HRP luminescent substrate is then added to detect the presence of antibody-bound cytokines. The cytokine concentration in each well is directly proportional to its luminescent intensity, which is measured as relative light units (RLU) by a microplate reader.
Data
