Characterization
Description(s):Cytokines are signaling molecules that have critical roles in many biological processes such as cellular growth, differentiation, gene expression, migration, immunity, and inflammation. Cytokines are secreted from cells and bound to cell-surface receptors, which initiate the activation of signal transduction pathways and mediate cell to cell communication. The malfunction of cytokines leads to many diseases, including arthritis, acute and chronic liver disease, inflammatory bowel disease, cardiac-related diseases, and cancers. A group of cytokines commonly involves in one biological or disease process. Therefore, the comprehensive analysis of the expression of multiple cytokines allows revealing the underneath mechanism of the disease state effectively. The Rat Cytokine ELISA Plate Array allows you to monitor the abundance of 16 rat cytokines in a high-throughput manner. This assay is a fast and sensitive tool for quantitatively profiling the levels of multiple cytokines between samples simultaneously.
Rat is the most chosen animal model for biomedical research of human disease due to a few reasons; physiologically closer to humans, larger sample volumes, easier surgeries, and faster to generate. Signosis provide Rat Cytokine ELISA Array for analyzing 16 most common rat cytokine expression in rat tissue, serum, plasma, cell culture medium, and cell lysate. The procedure is simple so that you can successfully conduct the assay at the first trial. We have Colorimetric and Chemiluminescence detection methods for your selection.
Detection Range:
8 - 2000 pg/mL
Sensitivity:
5 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | TNFa | IL-1a | TNFa | IL-1a | TNFa | IL-1a | TNFa | IL-1a | TNFa | IL-1a | TNFa | IL-1a |
| B | VEGF | IL-1b | VEGF | IL-1b | VEGF | IL-1b | VEGF | IL-1b | VEGF | IL-1b | VEGF | IL-1b |
| C | FGFb | IL-5 | FGFb | IL-5 | FGFb | IL-5 | FGFb | IL-5 | FGFb | IL-5 | FGFb | IL-5 |
| D | IFNy | IL-6 | IFNy | IL-6 | IFNy | IL-6 | IFNy | IL-6 | IFNy | IL-6 | IFNy | IL-6 |
| E | Leptin | IL-15 | Leptin | IL-15 | Leptin | IL-15 | Leptin | IL-15 | Leptin | IL-15 | Leptin | IL-15 |
| F | MCP-1 | IP-10 | MCP-1 | IP-10 | MCP-1 | IP-10 | MCP-1 | IP-10 | MCP-1 | IP-10 | MCP-1 | IP-10 |
| G | SCF | Rantes | SCF | Rantes | SCF | Rantes | SCF | Rantes | SCF | Rantes | SCF | Rantes |
| H | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb | MIP-1a | TGFb |
Principle
The 96-well white plate is divided into 3, 4, or 6 sections, and each section has 4, 3, or 2 columns for one sample. In each section, 32 (Human Cytokine ELISA Plate Arrays), 24 (Mouse Cytokine ELISA Plate Array), or 16 (Rat Cytokine ELISA Plate Array) specific cytokine capture antibodies are coated on 32, 24, or 16 wells respectively. The sample, cell culture supernatants, cell lysates, tissue homogenates, serum, or plasma samples among others are incubated with the cytokine ELISA plate, and the captured cytokine proteins are subsequently detected with a cocktail of biotinylated detection antibodies. The test sample is allowed to react with a pair of antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The plate is further detected with HRP luminescent substrate or HRP substrate TMB. The level of expression for each specific cytokine is directly proportional to the luminescent or color intensity.
Data
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Analysis of Cytokine Protein Expression in TNFa-Treated and Untreated with Rat Cytokine ELISA Plate Array. CWSV-1 Rat liver cells were starved for 24 hours with serum-free medium, subsequently treated the cells with and without 20ng/ul TNF for 16 hours. The serum-free conditioned media were incubated on the plate for 1 hour. After incubating with detection antibody mix and HRP, the plate was detected with chemilumincent substrate by a plate reader. |
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Literature
View user manual
Citations
Neuronal driven pre-plaque inflammation in a transgenic rat model of Alzheimer's disease. CE Hanzel, A Pichet Binette, LSB Pimentel, MF Iulita. Neurobiol Aging. 2014 Oct;35(10):2249-62. doi: 10.1016/j.neurobiolaging.2014.03.026. Epub 2014 Mar 28.
Prenatal inhibition of the tryptophan-kynurenine pathway alters synaptic plasticity and protein expression in the rat hippocampus. Forrest CM, Khalil OS, Pisar M, Darlington LG, Stone TW. Brain Res. 2013 Apr;1504:1-15.
Interkingdom signaling induces Streptococcus pneumoniae biofilm dispersion and transition from asymptomatic colonization to disease. Laura R. Marksa, Bruce A. Davidsonb,c, Paul R. Knighta,b,c,d, Anders P. Hakanssona,d,e 23 July 2013 mBio vol. 4 no. 4 e00438-13. 23 July 2013 mBio vol. 4 no. 4 e00438-13.
Evaluation of the efficacy of cell and micrograft transplantation for full-thickness wound healing. Carla R. Kruse, Dharaniya Sakthivel, Indranil Sinha, Douglas Helm, Jens A. Sørensen, Elof Eriksson, Kristo Nuutila. Journal of Surgical Research, vol. 227, 2018, pp. 35–43., doi:10.1016/j.jss.2018.02.004.
Modulation of the Tryptophan Hydroxylase 1/Monoamine Oxidase‐A/5‐Hydroxytryptamine/5‐Hydroxytryptamine Receptor 2A/2B/2C Axis Regulates Biliary Proliferation and Liver Fibrosis During Cholestasis. Kyritsi, K., Chen, L., O’Brien, A., Francis, H., Hein, T.W., Venter, J., Wu, N., Ceci, L., Zhou, T., Zawieja, D. and Gashev, A.A., 2020. Hepatology, 71: 990-1008. doi:10.1002/hep.30880
Antioxidant resveratrol restores renal sodium transport regulation in SHR, Apurva A. Javkhedkar, Anees A. Banday. Physiological Reports Published 24 November 2015 Vol. 3 no. e12618 DOI: 10.14814/phy2.12618
