Description(s):

Fibroblast growth factor (FGF)-2, also known as basic FGF (FGFb), is a powerful stimulator of angiogenesis in vivo. It also plays an important role during prenatal developmental processes and postnatal growth, by promoting cellular proliferation and differentiation in vitro. 

Detection Range:
- 16 - 1000 pg/mL 

Sensitivity:
- 12 pg/mL

Principle

The ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a anti-rat cytokine antibody for immobilization on the microtiter wells and anti-rat cytokine antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules of the cytokine being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of the cytokine is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

 

 

Data

Literature

View user manual

Citations

Early TBI-Induced Cytokine Alterations are Similarly Detected by Two Distinct Methods of Multiplex Assay. Mukherjee S, Katki K, Arisi GM, Foresti ML, Shapiro LA.  Front Mol Neurosci. 2011;4:21. doi: 10.3389/fnmol.2011.00021. Epub 2011 Sep
 
Pentosan polysulfate: a novel therapy for the mucopolysaccharidoses. Schuchman EH, Ge Y, Lai A, Borisov Y, Faillace M, Eliyahu E, He X, Iatridis J, Vlassara H, Striker G, Simonaro CM. PLoS One. 2013;8(1):e54459. doi: 10.1371/journal.pone.0054459. Epub 2013 Jan 24.