Description(s):
Leptin plays a key role in regulating energy intake and energy expenditure, including the regulation of appetite and metabolism. Leptin is produced by adipose tissue and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin is also found to stimulate endothelial cell proliferation and angiogenesis.
Detection Range:
- 16 - 1000 pg/mL
Sensitivity:
- 8 pg/mL
Principle
The ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a anti-rat cytokine antibody for immobilization on the microtiter wells and anti-rat cytokine antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules of the cytokine being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of the cytokine is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.
Data
Literature
View user manualCitations
Early TBI-Induced Cytokine Alterations are Similarly Detected by Two Distinct Methods of Multiplex Assay. Mukherjee S, Katki K, Arisi GM, Foresti ML, Shapiro LA. Front Mol Neurosci. 2011;4:21. doi: 10.3389/fnmol.2011.00021. Epub 2011 Sep
Pentosan polysulfate: a novel therapy for the mucopolysaccharidoses. Schuchman EH, Ge Y, Lai A, Borisov Y, Faillace M, Eliyahu E, He X, Iatridis J, Vlassara H, Striker G, Simonaro CM. PLoS One. 2013;8(1):e54459. doi: 10.1371/journal.pone.0054459. Epub 2013 Jan 24.
