Product Name | Catalog # | Price | Qty | |
---|---|---|---|---|
Rat Oxidative Stress ELISA Strip (Chemiluminescence) | EA-1831 | $648 |
Characterization
Description(s):Oxidative stress can directly damage cell structures and may lead to cancer, inflammatory diseases, and aging. Interestingly, oxidative stress can also alter the expression levels of a variety of cytokines. Understanding the conditions that alter the expression of these cellular messengers is important for unraveling the mechanisms of these diseases and developing therapeutics. Signosis’s Oxidative Stress ELISA Strip Profiling Assay can simultaneously analyze 8 oxidative stress cytokines. Each well of the strip is coated with a specific capture antibody to detect corresponding cytokine in the sample. The expression levels of these cytokines can be quantitatively compared between samples.
Detection Range:
2 - 2000 pg/mL
Sensitivity:
1 - 10 pg/mL
Applicable Grid:
List of Applicable Cytokines
TNFα | TGFβ | MCP-1 | IL-1α | IL-1β | IL-6 | IL-15 | VEGF |
Principle
Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, eight different proteins can be measured simultaneously. The test sample initially reacts with the solid phase capture antibody, resulting in the cytokine being bound to the well. The wells are then washed to remove unbound proteins, and biotin-linked antibodies are added to the samples to bind to the cytokines. After washing away the unbound antibodies, Streptavidin-HRP conjugate is added to the sample to form a complex with the antibody-bound cytokines. After incubation, the wells are washed to remove unbound Streptavidin-HRP conjugate. HRP luminescent substrate is then added to detect the presence of antibody-bound cytokines. The cytokine concentration in each well is directly proportional to its luminescent intensity, which is measured as relative light units (RLU) by a microplate reader.
Literature
View user manualCitations
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Acute inflammatory responses of nanoparticles in an intra-tracheal instillation rat model.AL Armstead, VC Minarchick, DW Porter… - PloS one, 2015. Published: March 4, 2015 DOI: 10.1371/journal.pone.0118778.
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