Characterization
Description(s):Receptor tyrosine kinases (RTKs) play important roles in converting extracellular to intracellular signals.They are activated by the binding of specific growth factors, cytokines, or hormones, which results in autophosphorylation. Tyrosine autophosphorylation generates recruitment sites for downstream signaling proteins containing Src homology-2 (SH2). Binding of different SH2 domain-containing signaling proteins represents the activation of different signaling pathways. Therefore, the activation of signaling pathways can be assessed through examination of SH2 domain binding. Signosis provided SH2 Domain-Based RTK Profiling Plate Array, which allows assessing the activation of RTK signaling pathways through the examination of 46 SH2 domain binding simultaneously.
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Applicable Grid:
List of Arrayed SH2 Domains
| 1 | 2 | 3 | 4 | 5 | 6 | 1 | 2 | 3 | 4 | 5 | 6 | |
| A | 3BP2 FP-0001 | CHN1 FP-0015 | GRB10 FP-0030 | LNK FP-0044 | P1K3R1-D2 | RaLP FP-0067 | 3BP2 FP-0001 | CHN1 FP-0015 | GRB10 FP-0030 | LNK FP-0044 | P1K3R1-D2 | RaLP FP-0067 |
| B | ALX FP-0004 | CHN2 FP-0016 | GRB14 FP-0031 | LYN FP-0045 | P1K3R2-D2 | RIN1 FP-0068 | ALX FP-0004 | CHN2 FP-0016 | GRB14 FP-0031 | LYN FP-0045 | P1K3R2-D2 | RIN1 FP-0068 |
| C | BCAR3 FP-0006 | CRK FP-0018 | GRB2 FP-0032 | MATK FP-0046 | PLCG1-D1 FP-0058 | RASA1 | BCAR3 FP-0006 | CRK FP-0018 | GRB2 FP-0032 | MATK FP-0046 | PLCG1-D1 FP-0058 | RASA1 |
| D | BLK FP-0007 | CRKL FP-0019 | HCK FP-0036 | MIST FP-0047 | PLCG2-D2 FP-0061 | SH2D2A | BLK FP-0007 | CRKL FP-0019 | HCK FP-0036 | MIST FP-0047 | PLCG2-D2 FP-0061 | SH2D2A |
| E | BMX-3 FP-0009 | CTEN FP-0021 | ITK FP-0038 | NCK1 FP-0048 | PTPN6-D1 FP-0065 | SH2D4A | BMX-3 FP-0009 | CTEN FP-0021 | ITK FP-0038 | NCK1 FP-0048 | PTPN6-D1 FP-0065 | SH2D4A |
| F | BRDG1 FP-0010 | FES FP-0025 | JAK1 FP-0039 | NCK2 FP-0049 | PTPN6-D2 FP-0066 | SHC FP-0078 | BRDG1 FP-0010 | FES FP-0025 | JAK1 FP-0039 | NCK2 FP-0049 | PTPN6-D2 FP-0066 | SHC FP-0078 |
| G | BTK FP-0011 | FYN FP-0028 | LCK FP-0042 | NSP1 FP-0050 | PTPNII-D1 FP-0063 | GST | BTK FP-0011 | FYN FP-0028 | LCK FP-0042 | NSP1 FP-0050 | PTPNII-D1 FP-0063 | GST |
| H | CBLC FP-0014 | GRAP FP-0029 | LCP2 FP-0043 | P1K3R1-D1 | PTPNII-D2 FP-0064 | Blank | CBLC FP-0014 | GRAP FP-0029 | LCP2 FP-0043 | P1K3R1-D1 | PTPNII-D2 FP-0064 | Blank |
Principle
SH2 domain-based RTK profiling Plate Array is an ELISA-like assay. SH2 domains are utilized for immobilization on the microtiter wells and an anti-phosphotyrosine antibody along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. Total 46 different SH2 domains are coated in different wells and 46 bindings can be measured. With a 96 well microplate, two samples can be compared among the bindings. The test sample is allowed to react simultaneously with the domain and the antibody. After incubation, the wells are washed to remove unbound-labeled antibody. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. Absorbance is measured spectrophotometrically at 450 nm.
Data
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