Characterization
Description(s):Stat3 (signal transducer and activator of transcription 3) promotes cell survival/proliferation, motility and immune tolerance and is considered as an oncogene. When signaled by cytokines and growth factors, such as oncostate, the activated Stat3 is translocated into the nucleus, where the gene expression is regulated by binding to the DNA recognition site. Signosis has developed a Stat3 ELISA kit to detect Stat3 activation. STAT1 exerts a wide spectrum of functions on both tumor cells and the immune system. Stat1 is usually considered as a tumor suppressor by enhancing inflammation and triggering anti-proliferative and pro-apoptotic responses in tumor cells. Upon activated by cytokines, such as INFgamma, stat1 forms homodimers or heterodimers with Stat3 or Stat2 and binds to the response element on the promoter region of target genes. Signosis has developed a Stat1 ELISA kit to monitor Stat1 activation.
Applicable Grid:
List of Applicable Transcription Factors
Principle
TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.
Data
Stat1 ELISA analysis. Hela cells were treated with and without 10ng/ml IFNgamma for 1 hour, then the nuclear extracts were prepared and subjected to Stat1 ELISA kit
