Description(s):

Stat5 EMSA (Electrophoretic-Mobility Shift Assay) kit is used for monitoring Stat5 (Signal transducer and activator of transcription 5) DNA binding activities and activation. Upon activation, Stat5 can recognize and interact with the unique DNA binding sequence and form protein/DNA complex, which can be separated from free DNA due to the differences in their electrophoretic mobility in native (non-denaturing) polyacrylamide gels.

Principle

When one TF is activated, it binds to a biotin-labeled probe, which is designed based on the consensus binding sequence of the TF. When the formed complex is subjected to electrophoresis, it runs slower than the free probe, and can be easily distinguished. Two or more samples can be compared and differences can be determined by the density of the shifted band.

Data

Literature

View user manual

Citations

 

P53 EMSA GS-0033

Piperlongumine exerts cytotoxic effects against cancer cells with mutant p53 proteins at least in part by restoring the biological functions of the tumor suppressor. D Basak, SR Punganuru, KS Srivenugopal .  International Journal of Oncology . Published online on: Wednesday, February 3, 2016. Print ISSN: 1019-6439 Online ISSN:1791-2423

Selective killing of human breast cancer cells by the styryl lactone (R)-goniothalamin is mediated by glutathione conjugation, induction of oxidative stress and marked reactivation of the R175H mutant p53 protein . Punganuru, Surendra R, et al.  Carcinogenesis, 2018, doi:10.1093/carcin/bgy093.

PR EMSA GS-0020

Enhanced Human Decidual Cell–Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal.F Schatz, O Guzeloglu-Kayisli, M Basar, LF Buchwalder… - The American Journal of Pathology Availabe online 21 July 2015

NFkB EMSA GS-0030

1. Perineural Dexmedetomidine Attenuates Inflammation in Rat Sciatic Nerve via the NF-κB Pathway. Yan Huang †, Yi Lu †, Lei Zhang, Jia Yan, Jue Jiang and Hong Jiang * Int. J. Mol. Sci. 2014, 15, 4049-4059; doi:10.3390/ijms15034049

2. Spleen tyrosine kinase inhibition attenuates airway hyperresponsiveness and pollution-induced enhanced airway response in a chronic mouse model of asthma. Penton PC, Wang X, Amatullah H, Cooper J, Godri K, North ML, Khanna N, Scott JA, Chow CW. J Allergy Clin Immunol. 2013 Feb;131(2):512-20.e1-10.

3.Imipramine blue halts head and neck cancer invasion through promoting F-box and leucine-rich repeat protein 14-mediated Twist1 degradation.WH Yang, YH Su, WH Hsu, CC Wang, JL Arbiser. Oncogene,(10 August 2015) | doi:10.1038/onc.2015.291

4. CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages. Shuai Wang, Zewei Sun, Wenting Zhao, Zhen Wang, Mingjie Wu, Yanyun Pan, Hui Yan, and Jianhua Zhu. Mediators of Inflammation, 2016, Article ID 1605948

5. Myeloid-derived interleukin-1β drives oncogenic KRAS-NF-κΒ addiction in malignant pleural effusion. Marazioti, Antonia, et al. Nature Communications, vol. 9, no. 1, 2018, doi:10.1038/s41467-018-03051-z.

6. Methods to Quantify the NF-ΚB Pathway During Senescence. Zhang, Lei, et al. Methods in Molecular Biology Cellular Senescence, 2018, pp. 231–250., doi:10.1007/978-1-4939-8931-7_18.

7. Transcriptional Regulation of CCL2 by PARP1 Is a Driver for Invasiveness in Breast Cancer.  Pranabananda Dutta, Kimberly Paico, Gabriela Gomez, Yanyuan Wu, Jaydutt V. Vadgama.  Cancers 2020, 12(5), 1317; https://doi.org/10.3390/cancers12051317

NFkB and CREB EMSA kit GS-0010 and GS-0030

1. Theophylline regulates inflammatory and neurotrophic factor signals in functional recovery after C2-hemisection in adult rats. Singh LP, Devi TS, Nantwi KD. Exp Neurol. 2012 Nov;238(1):79-88.

2. Protein kinase C inhibitor AEB071 targets ocular melanoma harboring GNAQ mutations via effects on the PKC/Erk1/2 and PKC/NF-κB pathways. Wu X, Li J, Zhu M, Fletcher JA, Hodi FS. Mol Cancer Ther. 2012 Sep;11(9):1905-14. doi: 10.1158/1535-7163.MCT-12-0121. Epub 2012 May 31.

3. Genetic deletion of CD36 enhances injury after acute neonatal stroke. Woo M, Wang X, Faustino JV, Derugin N, Wendland MF, Zhou P, Costantino Iadecola, Zinaida S. Vexler. Ann Neurol. 2012 Dec;72(6):961-70.

4. Myeloid-derived interleukin-1β drives oncogenic KRAS-NF-κΒ addiction in malignant pleural effusion. Marazioti, Antonia, et al. Nature Communications, vol. 9, no. 1, 2018, doi:10.1038/s41467-018-03051-z.

C/EBP and HSF GS-0006 and GS-0054

Glutamine-mediated Dual Regulation of Heat Shock Transcription Factor-1 Activation andExpression. Xue H, Slavov D, Wischmeyer PE.. J Biol Chem. 2012 Nov 23;287(48):40400-13.

TCF/LEF EMSA GS-0048

The SxxSS motif of T-cell factor-4 isoforms modulates Wnt/β-catenin signal activation in hepatocellular carcinoma cells. Tomimaru Y, Koga H, Shin TH, Xu CQ, Wands JR, Kim M. Cancer Lett. 2013 Apr 2. pii: S0304-3835(13)00273-5. doi: 10.1016/j.canlet.2013.03.031. [Epub ahead of print]

GR EMSA GP-0019

26S proteasome inhibitors inhibit dexamethasone-dependent increase of tyrosine aminotransferase and tryptophan 2,3-dioxygenase mRNA levels in primary cultured rat hepatocytes. Mizuho H, Masashi H, Youko N, Chieko S, Minako F, Taiichiro S, Toyohiko A, Nana K, Shingo N. Biophys Chem. 2012;3(4):348-356.

CAR  EMSA GS-007

Excessive hepatomegaly of mice with hepatocyte-targeted elimination of integrin linked kinase following treatment with 1,4-bis [2-(3,5-dichaloropyridyloxy)] benzene.Donthamsetty S, Bhave VS, Kliment CS, Bowen WC, Mars WM, Bell AW, Stewart RE, Orr A, Wu C, Michalopoulos GK. Hepatology.  2011 Feb;53(2):587-95.

YY1 EMSA GS-0051

Effect of a Single-Nucleotide Polymorphism in the Cholecystokinin Type A Receptor Gene on Growth Traits in the Hinai-dori Chicken Breed. Rikimaru K, Takeda H, Uemoto Y, Komatsu M, Takahashi D, Suzuki K, Takahashi H J. Poult. Sci., 2013 50: 206-211.

GR/PR EMSA GS-0019

Decreased levels of nuclear glucocorticoid receptor protein in the hippocampus of aged Long-Evans rats with cognitive impairment. Lee SY, Hwang YK, Yun HS, Han JS. Brain Res. 2012 Oct;1478:48-54.

HIF EMSA GS-0021

TXNIP Links Innate Host Defense Mechanisms to Oxidative Stress and Inflammation in Retinal Muller Glia under Chronic Hyperglycemia: Implications for Diabetic Retinopathy. Devi TS, Lee I, Hüttemann M, Kumar A, Nantwi KD, Singh LP. Exp Diabetes Res. 2012 ;2012:438238.

Custom HIF EMSA kit

Critical role of TXNIP in oxidative stress, DNA damage and retinal pericyte apoptosis under high glucose: implications for diabetic retinopathy. Devi TS, Hosoya KI, Terasaki T, Singh LP. Exp Cell Res. 2013 Jan 24. pii: S0014-4827(13)00019-0.

HOXA-5 EMSA Kit GS-0061

Nuclear complex of glyceraldehyde-3-phosphate dehydrogenase and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxidant-induced cell death. Xuwei Hou, Patricia Snarski, Yusuke Higashi, Tadashi Yoshida, Alexander Jurkevich, Patrick Delafontaine, and Sergiy Sukhanov. The FASEB Journal. 2017 April. DOI: 10.1096/fj.201601082R.

STAT3 EMSA Kit GS-0044

Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia. Jiang, Xi, et al.  Nature Communications, vol. 8, no. 1, 2017, doi:10.1038/s41467-017-02290-w.

Gong-Ping Liu, Xiao-Yue Hong, Hua-Li Wan et al. STAT3 ameliorates cognitive deficits by positively regulating the expression of NMDARs in a mouse model of FTDP-17, 07 May 2020, PREPRINT (Version 1) available at Research Square [+https://doi.org/10.21203/rs.3.rs-25793/v1+]

STAT3 ameliorates cognitive deficits via regulation of NMDAR expression in an Alzheimer's disease animal model. Wan HL, Hong XY, Zhao ZH, et al. Theranostics. 2021;11(11):5511-5524. Published 2021 Mar 13. doi:10.7150/thno.56541

STAT1 EMSA Kit GS-0043

Tau Accumulation Activates STAT1 Triggering Memory Deficits via Suppressing NMDA Receptor Expression. Li, Xiaoguang, et al. 2018, doi:10.1101/437814.

NRF2(ARE) EMSA Kit  GS-0031

Post-ischemic administration of 5-methoxyindole-2-carboxylic acid at the onset of reperfusion affords neuroprotection against stroke injury by preserving mitochondrial function and attenuating oxidative stress. Jinzi Wua, Zhen Jina, Xiaorong Yanga, b, Liang-Jun Yana. Biochemical and Biophysical Research Communications, 2018, doi:10.1016/j.bbrc.2018.02.106.

E2F-1 EMSA Kit  GS-0011

Involvement of pRb-E2F pathway in green tea extract-induced growth inhibition of human myeloid leukemia cells. Henry, Darrell, et al. Leukemia Research, 2019, doi:10.1016/j.leukres.2018.12.014.

GAS/ISRE EMSA Kit GS-0017

An Essential Role for Perforin-2 in Type I IFN Signaling. Ryan McCormack, Richard Hunte, Eckhard R. Podack, Gregory V. Plano, Noula Shembade. The Journal of Immunology. March 11, 2020, ji1901013; DOI: 10.4049/jimmunol.1901013

Stat6 EMSA Kit GS-0047

Channeling macrophage polarization by rocaglates increases macrophage resistance to Mycobacterium tuberculosis.  Chatterjee, S., Yabaji, S.M., Rukhlenko, O.S., Bhattacharya, B., Waligurski, E., Vallavoju, N., Ray, S., Kholodenko, B.N., Brown, L.E., Beeler, A.B., Ivanov, A.R., Kobzik, L., Porco Jr., J.A., Kramnik, I., ISCIENCE (2021), doi: https://doi.org/10.1016/j.isci.2021.102845.