How to order this kit:
Step 1: Choose filter plate assay
| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| TF Filter Plate Assay without Probe (Use with PP-xxxx) | FA-0010 | $484 |
Step 2: Choose probe of interest
| Product Name | Catalog # | Price | Qty | |
|---|---|---|---|---|
| NFκB | PP-0001 | $153 | ||
| HIF | PP-0002 | $153 | ||
| ER | PP-0003 | $153 | ||
| AP1 | PP-0004 | $153 | ||
| Stat3 | PP-0005 | $153 | ||
| AP2 | PP-0007 | $153 | ||
| AR | PP-0008 | $153 | ||
| ATF2 Probe | PP-0009 | $153 | ||
| Brn-3 | PP-0010 | $153 | ||
| C/EBP Probe | PP-0011 | $153 | ||
| CBF | PP-0012 | $153 | ||
| CAR | PP-0013 | $153 | ||
| CDP | PP-0014 | $153 | ||
| CREB | PP-0015 | $153 | ||
| E2F-1 | PP-0016 | $153 | ||
| EGR | PP-0017 | $153 | ||
| Ets | PP-0018 | $153 | ||
| FAST-1(FOXH1) | PP-0019 | $153 | ||
| GAS/ISRE | PP-0020 | $153 | ||
| GATA | PP-0021 | $153 | ||
| GR/PR | PP-0022 | $153 | ||
| HNF4 | PP-0023 | $153 | ||
| IRF | PP-0024 | $153 | ||
| MEF2 | PP-0025 | $153 | ||
| Myb | PP-0026 | $153 | ||
| Myc-Max | PP-0027 | $153 | ||
| NF-1 | PP-0028 | $153 | ||
| NFAT | PP-0029 | $153 | ||
| NF-E2 | PP-0030 | $153 | ||
| OCT4 | PP-0031 | $153 | ||
| p53 | PP-0032 | $153 | ||
| Pax-5 | PP-0033 | $153 | ||
| Pbx1 | PP-0034 | $153 | ||
| Pit | PP-0035 | $153 | ||
| PPAR | PP-0036 | $153 | ||
| PXR | PP-0037 | $153 | ||
| SMAD (MADH) | PP-0038 | $153 | ||
| Sp1 | PP-0039 | $153 | ||
| SATB1 | PP-0041 | $153 | ||
| Stat1 | PP-0042 | $153 | ||
| Stat4 | PP-0043 | $153 | ||
| Stat5 | PP-0044 | $153 | ||
| Stat6 | PP-0045 | $153 | ||
| TCF/LEF | PP-0046 | $153 | ||
| TFIID | PP-0047 | $153 | ||
| TR | PP-0048 | $153 | ||
| RB | PP-0081 | $153 | ||
| AP3 | PP-0049 | $153 | ||
| RNUX | PP-0082 | $153 | ||
| ROR (RZR) | PP-0083 | $153 | ||
| RXR | PP-0084 | $153 | ||
| SF1 | PP-0085 | $153 | ||
| AP4 | PP-0050 | $153 | ||
| SMUC | PP-0086 | $153 | ||
| Snail | PP-0087 | $153 | ||
| SOX2 | PP-0088 | $153 | ||
| SOX9 | PP-0089 | $153 | ||
| COUP | PP-0051 | $153 | ||
| SOX18 | PP-0090 | $153 | ||
| SRY | PP-0091 | $153 | ||
| TFE3 | PP-0092 | $153 | ||
| USF-1 | PP-0093 | $153 | ||
| ELK | PP-0052 | $153 | ||
| FOXA1 | PP-0053 | $153 | ||
| FoxC1 | PP-0054 | $153 | ||
| FOXD3 | PP-0055 | $153 | ||
| FOXG1 | PP-0056 | $153 | ||
| FOXO1 | PP-0057 | $153 | ||
| FREAC-2 (FOXF2) | PP-0058 | $153 | ||
| Gfi-1 | PP-0059 | $153 | ||
| Gli-1 | PP-0060 | $153 | ||
| HEN (NSCL-1) | PP-0061 | $153 | ||
| HNF-1 | PP-0062 | $153 | ||
| HOX4C | PP-0063 | $153 | ||
| HoxA-5 | PP-0064 | $153 | ||
| HSF | PP-0065 | $153 | ||
| KLF4 | PP-0066 | $153 | ||
| MyoD | PP-0067 | $153 | ||
| MZF | PP-0068 | $153 | ||
| Nkx2-5 | PP-0069 | $153 | ||
| Nkx3-2 | PP-0070 | $153 | ||
| NRF1 | PP-0071 | $153 | ||
| NRF2 (ARE) | PP-0072 | $153 | ||
| OCT1 | PP-0073 | $153 | ||
| Pax-2 | PP-0074 | $153 | ||
| Pax-3 | PP-0075 | $153 | ||
| Pax-8 | PP-0076 | $153 | ||
| PIT1 | PP-0077 | $153 | ||
| PLAG1 | PP-0078 | $153 | ||
| Prox1 | PP-0080 | $153 |
| Transcription factors (TFs) are a group of cellular proteins that play essential roles in regulating gene expression. They act as a sensor to monitor cellular change and convert the signals into altered gene expression. A specific TF is often regulated by multiple upstream proteins. Monitoring the activation of a TF is critical to uncovering the mechanism of signal pathways and cellular regulations. Signosis allows you to select up to 8 TFs of your interest to create a customized filter plate assay to better suit your needs. To create a customized filter plate assay, you simply order the core kit and choose your own combination of TF probes of interest! |
To perform the assay, a TF Filter Assay Kit and a probe are needed.
Principle
In Signosis' TF filter plate assays, a specific biotin-labeled TF DNA binding sequence is mixed with nuclear extracts to allow formation of TF-DNA complexes. A filter plate is used to retain bound DNA probe and remove free probe. The bound pre-labeled DNA probe is then eluted from the filter and collected for quantitative analysis through DNA plate hybridization. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer.
Data
Analysis of HeLa cells that were treated with TNFα, IL2, OSM, and IL4 with controls. The nuclear extracts were prepared and subjected to Filter profiling plate array testing for transcription factors STAT1, STAT3, STAT4, STAT5, and STAT6.
Literature
View user manualCitations
Citations
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2.Melatonin MT1 receptor-induced transcriptional up-regulation of p27Kip1 in prostate cancer antiproliferation is mediated via inhibition of constitutively active nuclear factor kappa B (NF-κB): potential implications on prostate cancer chemoprevention and therapy. Shiu SY, Leung WY, Tam CW, Liu VW, Yao KM. J Pineal Res. 2012 Jun 20. doi: 10.1111/j.1600-079X.2012.01026.x. [Epub ahead of print]
3.Deficiency of Capn4 Gene Inhibits Nuclear Factor-κB (NF-κB) Protein Signaling/Inflammation and Reduces Remodeling after Myocardial Infarction. Ma J, Wei M, Wang Q, Li J, Wang H, Liu W, Lacefield JC, Greer PA, Karmazyn M, Fan GC, Peng T. J Biol Chem. 2012 Aug 10;287(33):27480-9.
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7. In vivo selective imaging and inhibition of leukemia stem-like cells using the fluorescent carbocyanine derivative, DiOC5 (3). B Zhang, Y Shimada, J Kuroyanagi, M Ariyoshi . Biomaterials, 2015. Volume 52, June 2015, Pages 14–25
8. Repression of a chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouseJia-kuan Chena, 1, Wen-chen Wanga, 1, Li Zangb, Jie Zhaoc, Wei Lic, Tao Jianga, ,Biochemical and Biophysical , 2016. doi:10.1016/j.bbrc.2016.02.043
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10. Bmi1 inhibition enhances the sensitivity of pancreatic cancer cells to gemcitabine
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12. Adipose Tissue-specific Inhibition of Hypoxia-inducible Factor 1 Induces Obesity and Glucose Intolerance by Impeding Energy Expenditure in Mice. Zhang X, Lam KS, Ye H, Chung SK, Zhou M, Wang Y, Xu A. J Biol Chem. 2010 Oct 22;285(43):32869-77.
13. HIF-2α Suppresses p53 to Enhance the Stemness and Regenerative Potential of Human Embryonic Stem Cells. Das B, Bayat-Mokhtari R, Tsui M, Lotfi S, Tsuchida R, Felsher DW, Yeger H. Stem Cells. 2012 Aug;30(8):1685-1695.
14. Protective effects of tyrosol against LPS-induced acute lung injury via inhibiting NF-κB and AP-1 activation and activating the HO-1/Nrf2 Pathways. Wen-chen Wanga, Yan-min Xiaa , Bo Yangb, Xiang-ni Suc , Jia-kuan Chena, Wei Li, Tao Jiang. Biological & Pharmaceutical Bulletin Biological and Pharmaceutical Bulletin 2017 DOI:10.1248/bpb.b16-00756
15. Upregulation of miRNA-155 expression by OxLDL in dendritic cells involves JAK1/2 kinase and transcription factors YY1 and MYB. H Yan, S Wang, Z Li, W Zhao, Z Wang, Z Sun, Y Pan. International Journal of Molecular Medicine , Print ISSN: 1107-3756. Online ISSN:1791-244X, Published online on: Friday, March 11, 2016
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