Characterization

Description(s):

TFEB is a member of the basic Helix-Loop-Helix-Zipper family of transcription factors that specifically recognizes and binds E-box sequences (5'-CANNTG-3') on DNA. TFEB is a master gene for lysosomal biogenesis, and therefore plays a central role in regulating expression of lysosomal genes. TFEB also acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Under aberrant lysosomal storage conditions such as in lysosomal storage diseases, TFEB is translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes. Signosis has developed the TFEB ELISA kits for sensitive and specific analysis of the activities of TFEB in a high throughput manner. The kit can be used for human, mouse and rat samples.

Applicable Grid:

ELISA Kits Available:

TFEB ELISA Kit (Colorimetric) TE-0029

TFEB ELISA Kit (Chemiluminescence) TE-0030

Principle

TF ELISA kit is high sensitive and specific assay with a simple and optimized procedure. The 96-well (8X12 strip) clear plate is pre-immobilized with the TF consensus sequencing oligo. The activated TF in nuclear extract or the whole cell lysate is added in the well and binds to the oligo. The activated TF is detected with a specific antibody against the subunit and a HRP conjugated secondary antibody. The assay utilizes colorimetric detection method, which can be easily measured by spectrophotometry.

 

Data